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酸碱度对稻瘟病菌BAS4过表达菌株耐受性的影响
作者: 王长秘  王春梅  王云锋  刘林  刘艳琴  李成云  杨静*  
单位: (云南生物资源保护与利用国家重点实验室/农业生物多样性与病虫害控制教育部重点实验室/云南农业大学 植物保护学院  昆明650201)  
关键词: 稻瘟病菌  效应蛋白  水稻  形态发育指标  pH  
分类号:S432.1
出版年,卷(期):页码:2017 ,48 ( 10 ): 页码:1802-180
摘要:

【目的】探明酸碱度(pH)对稻瘟病活体寄生相关分泌蛋白BAS4(Biotrophy-associated secreted protein 4)过

表达菌株形态发育及受侵染水稻防御相关基因表达的影响,揭示pH对稻瘟病菌效应蛋白在稻瘟病菌与水稻互作中

的作用机制,为农业生产上预测预报不良环境因子对水稻生产的影响提供参考。【方法】利用不同pH(5.00、6.00、7.00

和8.00)处理稻瘟病菌过表达菌株35S:BAS4/Mo-1和野生型菌株A1343R-7,比较分析不同pH对过表达菌株菌落生长、

产孢量、孢子萌发率、菌丝干重和附着胞形成等形态发育的影响。调查pH 5.00和pH 8.00预处理过表达菌株再侵染

水稻的发病率,采用实时荧光定量PCR(qRT-PCR)分析受侵染水稻防御相关基因的表达情况。【结果】不同pH对BAS4

过表达菌株菌落生长、产孢量、萌发率、菌丝干重和附着胞形成的影响较pH对野生型菌株小,pH 5.00和pH 8.00处理

的BAS4过表达菌株均提高了其侵染水稻的发病率。pH 5.00和pH 8.00抑制其处理的BAS4过表达菌株侵染水稻病程

相关基因、SA-途径、JA-途径相关基因及PR5的表达,诱导HSP90在侵染水稻早期上调表达。【结论】pH可影响BAS4过

表达菌株的形态发育,但其处理的过表达菌株侵染水稻的大部分防御相关基因表达下调,预示BAS4过表达菌株对

pH具有较强的耐受性。


【Objective】This study investigated the effects of pH on morphological development of biotrophy-associated

secreted protein 4(BAS4)overexpressed strain and expression of defense-related genes in infected rice,and discovered

the mechanism of pH on rice blast effect protein in the interaction of rice blast strain and rice,in order to provide

important reference for predicting effects of adverse environmental factors on rice planting in agricultural production.【Method】Overexpressed strain of Magnaporthe oryzae(35S:BAS4/Mo-1)and wild strain A1343R-7(WT)were pretreated with different pH values(pH 5.00,pH 6.00,pH 7.00,and pH 8.00)to analyze the effects of pH on colony

growth,spore quantity,spore germination rate,dry weight of hypha,and appressorium formation of overexpressed

strain. And the disease incidence of overexpressed strain re-infected rice which were pre-treated with pH5.0 and pH8.0

was investigated and the expression of infected rice defense-related genes was analyzed by real-time fluorescent quantitative PCR(qRT-PCR).【Result】The result showed that the effect of pH on colony growth,spore quantity,dry weight of hypha,germination rate and appressorium formation of 35S:BAS4/Mo-1 was smaller than the effect on those of wildtype strain. Disease incidence of rice infected by overexpressed strains which were pre-treated with pH 5.00 and pH 8.00 was increased. pH 5.00 and pH 8.00 repressed the expression of pathogenesis-related genes,SA-pathway genes,JA-pathway genes and PR5 of overexpressed strain that infected rice,while up-regulated the expression of HSP90 in the early stage of infection in rice.【Conclusion】Although morphologic development of BAS4 overexpressed strains are influenced by pH,the expression of most rice denfense-related genes pre-treated with pH appears down-regulation,which indicates that BAS4 overexpressed strains have strong tolerance to pH.


基金项目:

国家自然科学基金项目(31400073);云南省自然科学基金面上项目(2013FB039);云南生物资源利用与保护国家重点实验室开放基金项目(KX141512)


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