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贵州辣椒脉斑驳病毒的检测及株系分化研究
作者: 王莉爽  陈小均  何海永  谭清群  陈 文  黄 露  杨学辉*  
单位: (贵州省植物保护研究所  贵阳 550006)  
关键词: 辣椒  辣椒脉斑驳病毒  株系分化  贵州  
分类号:S432.1
出版年,卷(期):页码:2017 ,48 ( 7 ): 页码:1220-1224
摘要:

【目的】明确贵州辣椒脉斑驳病毒(Chilli veinal mottle virus,ChiVMV)的分布和遗传进化情况,为该病毒的基因功能及其与宿主植物互作关系研究提供科学依据。【方法】采用反转录PCR(RT-PCR)对采自贵州贵阳、遵义、黔西和大方的疑似ChiVMV辣椒样品进行扩增并测序;运用DNAMAN和MEGA对ChiVMV CP基因和和3'-UTR进行序列拼接及分析,并构建系统发育进化树。【结果】从39份疑似ChiVMV辣椒样品中检测出7份阳性样品,检出率为17.95%。将贵州ChiVMV株系的部分CP基因和3'-UTR序列在NCBI中进行核苷酸序列比对,发现其与来源于四川辣椒上的ChiVMV株系的同源性最高,为99%,与我国其他省份ChiVMV株系同源性大于90%;构建的系统发育进化树表明贵州株系与四川和云南株系亲缘关系最近。【结论】贵州ChiVMV株系存在明显的株系分化现象。

【Objective】The present study aimed to define the distribution and evolution of Chilli veinal mottle virus(ChiVMV) in Guizhou, and provide scientific evidence for the interaction between gene function of the virus and host plant. 【Method】The reverse transcription PCR method(RT-PCR) was used to amplify the suspected ChiVMV pepper samples that collected from Guiyang, Zunyi, Qianxi and Dafang. Subsequently, the PCR products were amplified and sequenced. Sequence splicing and analysis of ChiVMV CP gene and 3'-UTR were performed by DNAMAN, and the phylogenetic tree was established by MEGA. 【Result】Seven positive samples were detected from thirty-nine samples of suspected ChiVMV peppers, the detection rate was 17.95%. Partial CP and 3'-UTR sequences of ChiVMV strain in Guizhou were compared with nucleotide sequence in NCBI. They showed the highest homology with the ChiVMV strain from Sichuan pepper, which was 99%. And their homology with ChiVMV strain in other provinces were above 90%. The phylogenetic tree also showed that the relationship between Guizhou strains, Sichuan strains, Yunnan strains were the closest. 【Conclusion】ChiVMV strains have obvious plant differentiation in Guizhou.

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