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超声辅助酶重量法提取树仔菜总膳食纤维
作者: 邓爱妮  范 琼  周 聪*  赵 敏  
单位: (中国热带农业科学院 分析测试中心/海南省热带果蔬产品质量安全重点实验室  海口 571101)  
关键词: 树仔菜  膳食纤维  酶重量法  超声辅助  
分类号:TS201.2
出版年,卷(期):页码:2017 ,48 ( 6 ): 页码:1062-1067
摘要:

【目的】优化超声辅助酶重量法提取树仔菜总膳食纤维工艺,为后续野生蔬菜膳食纤维研究工作提供技术参考。【方法】以树仔菜为原料,通过单因素试验考察蛋白酶种类、酶添加量、溶液pH、超声时间和超声温度对总膳食纤维提取率的影响,并在此基础上采用正交试验优化酶解工艺。【结果】蛋白酶种类对树仔菜总膳食纤维提取率影响的排序为木瓜蛋白酶>中性蛋白酶>碱性蛋白酶>胰蛋白酶;超声辅助酶重量法提取树仔菜总膳食纤维的最佳工艺条件为:木瓜蛋白酶添加量0.60%、溶液pH 5.0、超声时间50 min、超声温度65 ℃,对提取率影响程度排序为木瓜蛋白酶添加量>超声时间>超声温度>溶液pH;在优化条件下,树仔菜总膳食纤维提取率为45.0%,变异系数小于5.00%,测定结果与GB 5009.88-2014《食品中膳食纤维的测定》测定结果(44.8%)基本一致。【结论】超声辅助酶重量法提取树仔菜总膳食纤维具有酶解时间短、操作简便等优点,可用于批量样品总膳食纤维的提取。

【Objective】The extraction process of total dietary fiber from Sauropus androgynus L. Merr by ultrasonic-assisted enzymetic-gravimetric method was optimized, in order to provide a theoretical reference for researching dietary fiber of wild vegetable. 【Method】Using S. androgynus L. Merr as materials, the effects of protease types, enzyme amount, solution pH, ultrasonic time and ultrasonic temperature on extraction rate of total dietary fiber from S. androgynus L. Merr were investigated by single-factor test. Meanwhile, based on the results of single-factor test, the extraction technology was optimized using  orthogonal experiment. 【Result】Effects of protease types on extraction efficiency were ranked as follows: papain>neutral protease>alkaline protease>trypsin. The optimal technological parameters for ultrasound-assisted enzyma-
tic-gravimetric extraction of S. androgynus L. Merr total dietary fiber were as follows: papain amount 0.60%, solution pH 5.0, ultrasonic time 50 min and ultrasonic temperature 65 ℃. Extraction efficiency was effected by papain>ultrasonic time>ultrasonic temperature>solution pH. Under the above optimum conditions, the extraction rate of total dietary fiber from S. androgynus L. Merr reached up to 45.0%, which was close to the value(44.8%) of GB 5009.88-2014 Measurement of Dietary Fiber in Food. The coefficient of variation was less than 5.00%. 【Conclusion】The optimized extraction process by ultrasonic-assisted enzymetic-gravimetric method shortens enzymolysis time, and is easy to operate, hence it can be applied in scale extraction of total dietary fiber from samples.

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