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云南省部分烟区根结线虫形态和分子鉴定
作者: 杨艳梅  梁 艳  袁绍杰  赵希城  胡先奇*  
单位: (云南农业大学 省部共建云南生物资源保护与利用国家重点实验室  昆明 650201)  
关键词: 烟草  根结线虫  种类  云南  
分类号:S432.45
出版年,卷(期):页码:2017 ,48 ( 2 ): 页码:284-291
摘要:

【目的】明确当前云南省部分烟草种植区根结线虫的种类和分布,为烟草根结线虫病综合防治及烟草品种布局提供理论依据。【方法】2015年8~10月,在云南省主要烟草种植区昆明、曲靖和玉溪市采集54份烟草根结线虫病根样本,采用雌成虫会阴花纹形态观察及分子生物学检测(rDNA-ITS区和SCAR特异性鉴定)对病原根结线虫种类进行鉴定。【结果】采集的54份样本中有24份样本的病原为花生根结线虫(Meloidogyne arenaria),占44.44 %;7份样本为南方根结线虫(M. incognita),占12.96%;1份样本为爪哇根结线虫(M. javanica),占1.85%;11份样本为爪哇根结线虫和花生根结线虫的复合种群,占20.37%;5份样本为南方根结线虫和花生根结线虫的复合种群,占9.26%;3份样本为南方根结线虫和爪哇根结线虫的复合种群,占5.56%;3份样本为南方根结线虫、爪哇根结线虫和花生根结线虫3种线虫的复合种群,占5.56 %。【结论】当前危害云南省部分烟草种植区(昆明、曲靖和玉溪烟区)的根结线虫种类主要有南方根结线虫、爪哇根结线虫和花生根结线虫3种,以花生根结线虫为优势种群。

【Objective】The present study investigated species and distribution  of root-knot nematodes in tobacco growing regions of Yunnan, in order to provide reference for comprehensive prevention of root-knot nematodes disease and tobacco variety layout. 【Method】During August to October, 2015, 54 samples of root-knot nematode host on tobacco crops were collected from Kunming city, Qujing city and Yuxi city in Yunnan. The species of root-knot nematodes were identified by observing perineal patterns and molecular biological testing(PCR of ITS region of ribosomal DNA and sequence characterized amplified region, SCAR). 【Result】There were 24 samples belonging to Meloidogyne arenaria, accounting for 44.44%; seven M. incognita samples, accounting for 12.96%; one M. javanica sample, accounting for 1.85%. Eleven samples were compound population of M. javanica and M. arenaria, accounting for 20.37%. Five were compound population of M. incognita and M. arenaria, accounting for 9.26%. Three were compound population of M. incognita and M. javanica, accounting for 5.56%. Three belonged to compound population of M. incognita, M. javanica and M. arenaria, accounting for 5.56%. 【Conclusion】The species of root-knot nematodes in tobacco growing areas of Yunnan mainly include M. incognita, M. javanica and M. arenaria. M. arenaria is the dominant population.

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