<<登录旧系统审稿   登录新系统审稿>>

建议您登录自己的邮箱点击审稿链接进入审稿系统 ×
首页 学报新闻 学报概况 编委会 专家库 学术影响 投稿指南 审核流程 标准规范 下载专区 投诉邮箱 联系我们 EngLish

正文 您当前的位置:首页》正文

葡萄MrRPV1蛋白LRR结构域的功能研究
作者: 曲俊杰1  黄 羽2  卢 江1  3  尹 玲1 *  
单位: (1 广西作物遗传改良生物技术重点开放实验室  南宁 530007;2 广西农业科学院 葡萄与葡萄酒研究所  南宁 530007;  
关键词: 葡萄  霜霉病抗性蛋白(MrRPV1)  白粉病抗性蛋白(MrRUN1)  LRR结构域  抗病性  
分类号:S663.1
出版年,卷(期):页码:2017 ,48 ( 2 ): 页码:202-210
摘要:

【目的】明确葡萄霜霉病抗性蛋白(MrRPV1)LRR结构域对葡萄霜霉菌特异性识别的影响,为进一步研究葡萄与霜霉菌间的互作机理打下基础。【方法】将MrRPV1和白粉病抗性蛋白(MrRUN1)两个蛋白的LRR结构域互换,构建重组表达载体后转化至感霜霉病和白粉病的欧亚种西拉葡萄胚性愈伤组织中以获得转基因植株,并通过DNA、RNA分子水平检测和室内接种试验进行抗病性鉴定。【结果】共获得7个RGA10-8LRR阳性转基因(重组了MrRPV1蛋白LRR结构域)株系和6个RGA8-10LRR阳性转基因(重组了MrRUN蛋白LRR结构域)株系。在7个RGA10-8LRR阳性转基因株系中,仅RGA10-8-13阳性植株表现出与MrRPV1转基因苗S8-1一致的坏死斑点,肉眼观察不到白色霜霉状物,但7个阳性转基因系均未表现出明显的白粉病抗性。在6个RGA8-10LRR阳性转基因株系中,RGA8-10-3和RGA8-10-28两个株系表现出对白粉病的抗性,有35%左右的细胞表现出细胞程序性坏死(PCD);RGA8-10LRR转基因苗均不抗霜霉病。【结论】MrRPV1的LRR结构域可能介导对葡萄霜霉菌的特异性识别,而MrRUN1的LRR结构域可能介导对白粉病菌的特异性识别。

【Objective】The function of LRR domain of grape downy mildew resistance protein(MrRPV1) in downy mildew pathogen specific recognition was investigated in order to provide basis for studying the interaction mechanism between grape and downy mildew pathogen. 【Method】The LRR domains of  MrRPV1 and powdery mildew resistance protein(MrRUN1) were switched and recombinant expression plasmids were constructed in this study. The recombinant expression plasmids were transformed into embryonic callus of Shiraz grape which was susceptible to downy mildew and powdery mildew so as to obtain transgenic plants. Disease resistance of the transgenic plants was identified by DNA and RNA molecular level detection and indoor inoculation test. 【Result】In total, seven RGA10-8LRR(recombination of LRR domains of  MrRPV1) and six RGA8-10LRR(recombination of LRR domains of  MrRUN1) positive transgenic lines were obtained. Among the seven RGA10-8LRR transgenic lines, only RGA10-8-13 line exhibited similar necrotic spots to MrRPV1 transgenic plant S8-1 with no visible downy mildew. Furthermore, all the seven RGA10-8LRR transgenic lines showed no significant powdery mildew resistance. Powdery mildew resistance was observed in two RGA8-10LRR transgenic lines, namely RGA8-10-3 and RGA8-10-28, with programmed cell necrosis(PCD) in 35% cells. All the six RGA8-10LRR transgenic lines showed no downy mildew resistance. 【Conclusion】LRR domain of MrRPV1 may play a role in specific recognition of  grape downy mildew pathogen and LRR domain of MrRUN1 may play a role in specific recognition of powdery mildew pathogen.

基金项目:
作者简介:
参考文献:
服务与反馈:
文章下载】【加入收藏
总浏览数: 9 8 8 1 6 1 7  今日总人数: 4 8 4 0 1 5
主办:广西农业科学院    地址:广西南宁市大学东路174号   邮政编码:530007
电话:(+86)-771-3243905; (+86)-771-3244920   电子邮箱:nfnyxb@163.com
Copyright © 2011 Nfnyxb.com, All Rights Reserved  版权所有©2011《南方农业学报》编辑部   
 本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:400-921-9838

;