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24份古荔枝种质资源ISSR遗传多样性分析
作者: 陆贵锋1  黄 川2  刘 钰3  廖慧茜4  丁 峰5  罗红卫2  李冬波1  李鸿莉1  
彭宏祥1 *
  朱建华1  黄凤珠1  徐 宁1  秦献泉1  
单位: (1 广西农业科学院 园艺研究所  南宁 530007;2 灵山县农业科学研究所  广西 钦州 535400;3 玉林市农业科学研究所  
关键词: 古荔枝  ISSR分子标记  遗传多样性  聚类分析  
分类号:S667.1
出版年,卷(期):页码:2017 ,48 ( 2 ): 页码:197-201
摘要:

【目的】了解广西古荔枝种质资源的遗传多样性及其亲缘关系,为其种质资源利用和品种选育等提供参考。【方法】采用ISSR分子标记技术对24份古荔枝种质资源的遗传多性及亲缘关系进行分析。【结果】从100条ISSR引物中筛选出13条带形清晰、重复性好的引物,共扩增出96条条带,其中有43条为多态性条带,多态性比例为44.79%,平均每条引物扩增的条带数为7.4条。供试古荔枝种质资源间的遗传相似系数范围为0.43~1.00,平均相对遗传相似系数为0.61。UPGMA法聚类分析结果表明,在遗传相似系为0.66处供试的24份古荔枝种质资源被分为三大类群。类群Ⅰ均来自广西灵山县,类群Ⅱ来自广西桂平市、北流市、玉林市、灵山县和大新县,类群Ⅲ来自广西北流市和灵山县。【结论】24个广西古荔枝品种资源间的遗传基础宽,可作为今后荔枝杂交选育的优良种质资源。

【Objective】The present study explored the genetic diversity and relationships of Guangxi ancient litchi germplasm resources  in order to provide references for utilization of germplasm resources and variety breeding. 【Method】The genetic relationships of 24 ancient litchi varieties were analyzed using ISSR molecular marker. 【Result】Thirteen primers with clear bands and favorable repeatability were selected from 100 ISSR primers; 96 bands were amplified altogether and 43 of them were polymorphic bands with a polymorphism proportion of 44.79%, and 7.4 bands were amplified by each primer on average. The genetic similarity coefficients among the tested ancient litchi germplasm resources ranged from 0.43 to 1.00 with average relative genetic similarity coefficient of 0.61. UPGMA cluster analysis results indicated that, the 24 tested germplasm resources of ancient litchi could be classified into three groups when genetic similarity coefficient was 0.66. Group Ⅰ came from Lingshan county, Guangxi; Group Ⅱ derived from Guiping city, Beiliu cuty, Yulin city, Lingshan county and Daxin county in Guangxi; Group Ⅲ came from Beiliu city and Lingshan county in Guangxi. 【Conclusion】The hereditary basis among the 24 varieties of ancient litchi in Guangxi is extensive, and they can serve as excellent materials for cross breeding of litchi in the future.

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