【Objective】The present study researched effects of soil and foliar applications of selenium on rice yield， quality， distribution of selenium in seedling， in order to provide reference for selenium-enriched rice cultivation. 【Method】BoIIIyou and Sanxiang 628 were taken as experiment materials. After soil and foliar applications of sodium selenite on two rice varieties， yield， quality， the content distribution of selenium in the two rice varieties were determined. 【Result】Soil and foliar applications of selenium had no significant effect on rice biomass production and harvest index（P>0.05）， but they could increase rice grain protein content by 1.57% and 2.36% respectively. Significant difference on protein content was found between two varieties. Meanwhile， compared to control， soil application of selenium and foliar application of selenium significantly increased（P<0.05） the selenium content in rice grain by 5.48 and 10.84 times respectively. For both varieties， selenium content in rice grain in foliar application was the higher than those in soil application and control. Furthermore， after soil application of selenium， Sanxiang 628 contained higher proportion of selenium retention in roots than BoIIIyou， while Sanxiang 628 contained lower distribution proportion in stem， leaf and grain than BoIIIyou. After foliar application of selenium， Sanxiang 628 contained higher proportion of selenium in leaf than BoyIIIyou， but Sanxiang 628 contained lower proportion of selenium in root， stem and grain than BoIIIyou. These data indicated that selenium distribution differences in grain and other parts of the two rice varieties might an important cause of differences in grain selenium content. 【Conclusion】Agronomic measures like soil and foliar applications of selenium can regulate selenium accumulation in grain to a certain extent. In this way， selenium nutritional quality for people who eat rice as staple food can be improved.