|作者： 魏翠华 谢 宇 秦建彬 陈 沁|
|单位： （福州市农业科学研究所 福州 350018）|
|关键词： 台湾金线莲 组培苗规格 栽培基质 成活率 鲜重增殖倍数 折干率|
|出版年,卷(期):页码：2016 ,47 ( 1 )： 页码：92-95|
【Objective】The tissue-cultured plantlets transplanting size and cultivation medium of Anoectochilus formosanus were screened， in order to provide references for optimizing cultivation techniques of A. formosanus. 【Method】Taking A. formosanus plantlets with 5 sizes （smaller-sized plantlets， small-sized plantlets， medium-sized plantlets， big-sized plantlets and bigger-sized plantlets） as materials， the field-planting experiment was carried out to compare growth status of A. formosanus by using 7 kinds of cultivation media with different formulas. Meanwhile， the survival rate of plantlets in vitro after field-planting for 1 month and 6 months， the proliferation rate of fresh weight and the dry weight/fresh weight ratio（DW/FW） after field-planting for 6 months were determined. 【Result】The results showed that， the survival rate of A. formosanus plantlets with 5 sizes after field-planting reached up to 90.0%， the proliferation rate of fresh weight for small-sized plantlets were significantly higher than the big-sized ones， and A. formosanus plantlets with different sizes were ranked according to their proliferation rates of fresh weight， as follows: smaller-sized plantlets >small-sized plantlets> big-sized plantlets > medium-sized plantlets > bigger-sized plantlets. Moreover， the DW/FW of small and medium-sized plantlets were higher than big-sized ones， and A. formosanus plantlets with different sizes were ranked according to DW/FW， as follows: medium-sized plantlets > small-sized plantlets > smaller-sized plantlets > big-sized plantlets> bigger-sized plantlets. In addition， the plantlets planted in cultivation medium composed of aquatic weed had the maximal proliferation rate（1.96） of fresh weight and the minimal DW/FW（8.8%）， but the planting speed of plantlets in vitro was slow. The plantlets planted in cultivation medium composed of Klasmann peat 422# had the maximal survival rate（100.0%） and DW/FW（10.6%）， but the its cultivation cost was higher. However， the cultivation cost of plantlets planted in cultivation medium composed of forest humus soil was lower， and the cultivation effect were above average. 【Conclusion】The cultivation effect of small-sized A. formosanus plantlets in vitro was better than that of big-sized ones. And the cultivation media composed of Klasmann peat 422# and forest humus soil show better cultivation effect. Furthermore， the low-cost forest humus soil can be used as preferred cultivation medium of A. formosanus in the mountainous rural area.