【Objective】The regional adaptation and stability of new sugarcane varieties in Guangxi were evaluated， in order to provide technical references for certification， promotion and application of new sugarcane varieties. 【Method】Using AMMI model， the adaptation and stability of ratoon-cane yield trait in Guangxi regional trial of 2014 were analysed. Further， the hierarchical cluster analysis was conducted based on phenotypic value of yield trait and related stability parameters of tested varieties. 【Result】Among 11 tested varieties， GT06-244， GT06-1721， GT03-1438， GT07-994 and LC05-136 were higher in yield， but GT05-3626 and GT03-3005 were more superior in stability. GT03-1438， GT06-244， GT06-1721， LC05-136， GT07-994 and GT05-3626 had more adaptability in trial sites of Hechi， Liuzhou and Nanning. Moreover， GYC1-2003， GT06-2361， GT06-3283， GT07-645 and GT03-3005 had more adaptability in trial sites of Longzhou and Baise. Among 5 trial sites， Longzhou was the strongest in discriminability， but Liuzhou was the weakest. In addition， the hierarchical cluster analysis showed that， all tested varieties were clustered into in 5 clusters， as follows: Cluster Ⅰ included GT07-994， GT06-244 and GT06-1721， which were characterized by high yield and sugar content but ordinary stability. Cluster Ⅱ included LC05-136 and GT03-1438， which were characterized by very high yield or sugar content but with poor stability. Cluster Ⅲ included GYC1-2003， GT07-645 and GT06-3283， which were characterized by same yield with control variety and ordinary stability. Cluster Ⅳ included only GT06-2361， which was characterized by low yield and poor stability. Cluster Ⅴ included GT05-3626 and GT03-3005， which were characterized by low yield and strong stability. 【Conclusion】GT06-244， GT07-994， GT06-1721， LC05-136 and GT06-3283 show better comprehensive yield-trait performance， which should be demonstrated， promoted and applied according to local conditions and variety characteristics.