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葡萄芪合酶基因家族上游调控序列分析
作者: 王超霞  张雅丽  卢 江*  
单位: (中国农业大学 食品科学与营养工程学院  北京 100083)  
关键词: 葡萄  芪合酶  基因  顺式作用元件  上游调控序列  
分类号:S663.1
出版年,卷(期):页码:2016 ,47 ( 1 ): 页码:1-6
摘要:

【目的】通过预测葡萄芪合酶(Stilbene synthase,STS)基因上游调控序列的顺式作用元件及分析不同STS基因上游调控序列,为进一步揭示STS基因在葡萄生长发育及胁迫应答过程中的作用提供理论依据。【方法】通过PlantCARE数据库,在线对欧洲种葡萄PN40024全基因组里的48条STS基因上游调控序列及目前已报道来自其他葡萄品种(圆叶葡萄Noble、华东葡萄白河35-1和欧亚种葡萄无核白、红地球、赤霞珠等)的8条STS基因启动子序列进行顺式作用元件分析。【结果】STS基因上游调控区域含有多种顺式作用元件,可以分为过程特异元件、诱导子特异元件、结合位点特异元件、环境特异元件、植物组织特异元件、调控特异元件、光响应元件及转录相关元件八大类,其中与抗病相关且出现次数较多的元件有TCA-element、ABRE、ERF、P-box、Box-W1、TC-rich repeats等。STS基因上游调控序列中含有数量不同的作用元件,其中欧亚种佳丽酿VvPinSTS26上游调控序列含有的顺式作用元件数量最多(49个),而VvPinSTS24上游调控序列含有的顺式作用元件数量最少(2个)。【结论】葡萄STS基因家族大部分成员的表达受水杨酸、脱落酸、赤霉素、乙烯、真菌诱导子等因素影响,从而调节白藜芦醇参与葡萄生物胁迫或非生物胁迫的防御过程。

【Objective】In order to provide theoretical basis for studying effect of stilbene synthase(STS) gene on grape growth and stress-response mechanisms, the present experiment aimed to investigate the cis-acting regulation elements in promoter region of STS genes and upstream regulatory sequences of STS genes from different organisms. 【Method】Based on genome sequence of Vitis vinifera‘PN40024’, 48 STS family genes was predicted. In addition, the reported STS genes from 8 grape cultivars viz., Muscadine rotundifolia ‘Noble’, Vitis pseudoreticulata ‘Baihe 35-1’ and V. vinifera ‘Thompson Seedless’, ‘Red Globe’ and ‘Cabernet Sauvignon’ were also selected. Then, the cis-acting elements in upstream regulatory sequences of those STS genes were analyzed using PlantCARE database. 【Result】The result showed that, there existed many cis-acting elements in upstream regulatory sequences of STS genes, which could be classified into 8 categories viz., component specific, elicitor specific, binding site specific, condition specific, plant tissues specific, regulation specific, light responsiveness and transcription elements. Of which, some elements such as TCA-element, ABRE, ERF, P-box, Box-W1 and TC-rich repeats were related to grape resistance. Furthermore, there were differences in the number of cis-acting elements in upstream regulatory sequences between these STS genes, as follows: the upstream regulatory sequence of V. vinifera ‘Carinena’ VvPinSTS26 gene had the most number of cis-acting elements(49), while VvPinSTS24 gene had the fewest number of cis-acting elements(only 2 ones). 【Conclusion】Based on above-mentioned results, it can be inferred that the STS gene expression is affected by ABA, SA, GA, ethylene and fungal elicitor, so that these elicitors play a role in regulating resveratrol synthesis in the grape defense process.

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