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4种处理方法对月季种子萌发的影响
作者: 闫海霞  蒋月喜  黄昌艳  何荆洲  王晓国  邓杰玲  卜朝阳*  
单位: (广西农业科学院 花卉研究所  南宁 530007)  
关键词: 月季  种子萌发  无菌萌发  发芽率  
分类号:S685.12
出版年,卷(期):页码:2016 ,47 ( 12 ): 页码:2108-2112
摘要:

【目的】分析不同处理方法对月季种子萌发的影响,为有效提高月季种子发芽率及育种效率提供理论和技术支持。【方法】以月季种子为材料,开展低温层积处理、不同激素及浓度、不同播种基质及无菌播种对其萌发影响的研究。【结果】低温层积处理的月季种子发芽率为42.22%,发芽势为24.44%;100.0 mg/L GA3和100.0 mg/L NAA处理可显著提高月季种子萌发率和发芽势(P<0.05),发芽率分别为55.56%和48.89%,发芽势分别为42.22%和30.00%;月季种子在河沙中发芽率为42.22%,发芽势为24.44%,优于泥炭∶珍珠岩(体积比为2∶1)及菜园土基质;在MS培养基中添加GA3更有利于月季种子萌发,当GA3浓度为0.5 mg/L时,其发芽率为55.56%,发芽势为34.44%。【结论】低温层积处理、100.0 mg/L GA3和100.0 mg/L NAA浸泡月季种子可有效提高其萌发率;适宜的播种基质及培养基对月季种子的萌发有促进作用。

【Objective】The present study was conducted to analyze effects of different treatments on germination of Rosa chinensis seeds in order to provide a theoretical and technical support for raising seed germination rate and breeding efficiency of R. chinensis. 【Method】Using R. chinensis seeds as material,the effects of cold stratification, different hormone concentrations, different substrates and aseptic seeding on seed germination were studied. 【Result】Results showed that: after cold stratification treatment, seed germination rate reached 42.22% and germination energy was 24.44%;100.0 mg/L GA3 and 100.0 mg/L NAA could significantly raise germination rate and germination energy of R. chinensis(P<0.05). After 100.0 mg/L GA3 treatment, seed germination rate was 55.56% and germination energy 42.22%; in 100.0 mg/L NAA treatment, seed germination rate reached 48.89% and germination energy 30.00%. In terms of R. chinensis planted in river sand, the germination rate was 42.22% and germination energy 24.44%, which were better than those in peat-perlite matrix (volume ratio 2∶1) and garden soil. Adding GA3 in MS media was beneficial for seed germination. When the concentration of GA3 was 0.5 mg/L, seed germination rate reached 55.56% and germination energy 34.44%. 【Conclusion】Cold stratification, 100.0 mg/L GA3 and 100.0 mg/L NAA soaking treatments can raise seed germination rate of R. chinensis. Appropriate seeding matrix and culture medium can promote seed germination of R. chinensis.

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