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不同素质烟叶烘烤过程中颜色值与色素含量的关系
作者: 李生栋1  谭方利2  黎妍妍3  黄克久4  吴文信2  郭保银4  陈少鹏4  宋朝鹏1 *  
单位: (1 河南农业大学 烟草学院  郑州 450002;2 湖南省烟草公司 郴州市公司  湖南 郴州 423000;3 湖北省烟草科学研究院  
关键词: 烟叶素质  颜色参数  色素含量  
分类号:S572.092
出版年,卷(期):页码:2016 ,47 ( 9 ): 页码:1570-1575
摘要:

【目的】研究不同素质烟叶烘烤过程中外观颜色参数与内在色素含量间的关系,为实现不同素质烟叶的精准烘烤提供理论依据。【方法】以烟叶K326品种为试验材料,分别取其下部嫩黄烟、中部返青烟、上部高温逼熟烟和上部贪青晚熟烟4种特殊素质烟叶进行烘烤,测定烘烤过程中烟叶外观颜色参数[绿色度(SPAD)、明度值(L*)、红绿值(a*)、黄蓝值(b*)]及色素含量,并对二者进行相关分析。【结果】4种素质烟叶的SPAD均在30~38 ℃下降最快。烘烤过程中,4种素质烟叶的L*、a*和b*均呈不同程度上升趋势,返青烟、嫩黄烟和高温逼熟烟在30~38 ℃上升最快,贪青晚熟烟在30~42 ℃上升最快。叶绿素a与叶绿素b的含量变化趋势基本一致,嫩黄烟、返青烟和高温逼熟烟在30~38 ℃降解量最大,贪青晚熟烟则在42~48 ℃降解量最大;4种素质烟叶的类胡萝卜素含量相对降解量均较小,其中嫩黄烟的类胡萝卜素含量在42~48 ℃迅速下降,高温逼熟烟在30~38、48~54 ℃时有较小幅度下降,返青烟和贪青晚熟烟则呈缓慢下降趋势;嫩黄烟、返青烟、高温逼熟烟和贪青晚熟烟的类胡萝卜素含量与叶绿素含量比值增长最快分别出现在30~42、30~38、42~48和48~54 ℃。不同素质烟叶SPAD与叶绿素含量均呈极显著正相关(P<0.01,下同),高温逼熟烟L*与叶绿素a含量呈显著负相关(P<0.05),其他3种素质烟叶L*、a*、b*与叶绿素含量均呈极显著负相关。【结论】不同素质烟叶在烘烤过程中其外观颜色参数与内在色素含量具有密切相关性,可依据外观颜色参数估计烟叶内部色素含量,为准确把握不同素质烟叶烘烤进程提供理论依据。

【Objective】The present study was conducted to study relationship between color parameters and pigment contents of different-quality tobacco leaves, in order to provide theoretical foundation for precise flue curing of different-quality tobacco. 【Method】Tobacco K326 was chosen as the experiment material. Four special quality tobacco namely tender leaves at bottom part, re-greening leaves at middle part, heat-forced maturity leaves at top part and over-green and late-maturing leaves at top part were flue cured. During the procedure, color parameters[soil and plant analyzer development value(SPAD), lightness value(L*), red-green index(a*), yellow-blue index(b*)] and pigment content of tobacco were measured and analyzed. 【Result】SPAD of four kinds of leaves dropped at fastest speed during 30-38 ℃. During flue curing process, L* , a*, b* of four kinds of leaves increased to different extent. The values of re-greening leaves, tender leaves, heat-forced maturity leaves increased most rapidly during 30-38 ℃ and those of over-green and late-maturing leaves increased most quickly during 30-42 ℃. Variation trends of chlorophyll-a and chlorophyll-b contents were similar. During 30-38 ℃, degradation amount of tender leaves, re-greening leaves and heat-forced maturity leaves reached maximum, while over-green and late-maturing leaves reached degradation amount peak during 42-48 ℃. Degradation amount of carotenoids contents of four kinds of leaves were relatively small. In terms of tender leaves, carotenoids contents dropped rapidly during 42-48 ℃; carotenoids contents of heat-forced maturity leaves dropped slightly both during 30-38 and 48-54 ℃; carotenoids contents of re-greening leaves and over-green and late-maturing leaves went through a slow decline. The ratio of carotenoids contents to chlorophyll contents of tender leaves, re-greening leaves, heat-forced maturity leaves and over-green and late-maturing leaves increased during 30-42, 30-38, 42-48 and 48-54 ℃, respectively. There was extremely significant positive correlation between SPAD and chlorophyll content in four kinds of tobacco leaves(P<0.01, the same below). There was significant negative correlation between L* and chlorophyll-a in heat-forced maturity leaves(P<0.05). In terms of other 3 kinds of leaves, there was extremely significant negative correlation between L*, a*, b* and chlorophyll contents. 【Conclusion】During flue curing process, color parameters and pigment contents of different-quality tobacco leaves are closely related. Color parameters can be used to estimate pigment contents of tobacco, in order to provide theoretical foundation for handling flue-curing process of different quality tobacco.

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