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猪嵴病毒VP1基因序列测定与分析
作者: 易春华1  谢齐斌1  唐薇薇1  朱春刚1  秦长串2  伍和明1  韦达有1 *  
单位: (1 桂林市动物疫病预防控制中心  广西 桂林 541001;2 临桂县动物疫病预防控制中心  广西 临桂 541007)  
关键词: 猪嵴病毒  VP1基因  序列测定  分析  
分类号:S852.659.6
出版年,卷(期):页码:2016 ,47 ( 4 ): 页码:670-673
摘要:

【目的】对猪嵴病毒(PKV)VP1基因进行测序与同源性分析,确认其可能来源,为今后的生物学特性分析及仔猪腹泻防治工作提供科学依据。【方法】采用RT-PCR对GXPKV-1毒株VP1基因进行克隆,运用DNASTAR软件包中Megalign程序对测序获得的VP1基因进行核苷酸序列及其推导氨基酸序列同源性分析。【结果】GXPKV-1毒株VP1基因全长762 bp,共编码254个氨基酸,与GenBank已公布的13株参考毒株VP1基因的核苷酸同源性为74.1%~85.4%,推导氨基酸同源性为81.1%~93.3%。根据VP1基因推导氨基酸序列进行系统发育进化分析,发现GXPKV-1毒株与Gansu-2012、JS1419等国内参考毒株同属于同一亚群,而与瑞士分离株Swine-S-1-2007、泰国分离株THA-2008、美国分离株H24-2012-USA及四川分离株CHN-SC-2011-02等的亲缘关系较远。【结论】猪嵴病毒GXPKV-1毒株起源于国内流行毒株的传播,在新的环境下虽然其VP1基因核苷酸发生变异,但由于同义翻译,推导氨基酸的同源性仍然较高,说明碱基突变并未引起蛋白结构的改变。

【Objective】The present experiment was conducted to analyze sequence and homology of VP1 gene of procine kobuvirus(PKV), and ascertain its possible source, in order to provide scientific basis for biological characteristics analysis and prevention of piglet diarrhea in future. 【Method】The VP1 gene was cloned from strain GXPKV-1 using RT-PCR technique. The homology of its nucleotide sequence and the deduced amino acid sequence was analysed by using Megalign program in DNASTAR software packages. 【Result】The results showed that, the GXPKV-1 VP1 gene was 762 bp in length, encoding 254 amino acids. The VP1 gene of GXPKV-1 shared nucleotide homology of 74.1%-85.4% and amino acid homology of 81.1%-93.3% with those of 13 strains published in GenBank. The phylogenetic evolution analysis showed that, GXPKV-1 stain, Gansu-2012 and JS1419 belonged to same subgroup, and GXPKV-1 stain had distant genetic relationship with Swiss isolates Swine-S-1-2007, Thailand isolates THA-2008, United States isolates H24-2012-USA and Sichuan isolates CHN-SC-2011-02. 【Conclusion】The GXPKV-1 strain is originated in the spread of pandemic strain in China. Under the new environment, the nucleotides of PKV VP1 gene mutate, however most of which is synonymous mutation, so the homology of amino acid are still high, it indicates that base variation doesn’t cause change of protein structure.

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