【Objective】By using different types of explants， tissue culture of purple-heart potato variety Black Beauty was studied in order to provide technical supports for its factory production in Guangxi. 【Method】The terminal buds，the different section axillary buds of potato variety Black Beauty were used as different explants， and MS was used as base medium. The effects of 0.1% HgCl2（Mercuric chloride） sterilizing time on explants were investigated， as well as 6-BA on primary culture， different concentrations of 6-BA combined with PP333 on proliferation culture， NAA on rooting and sugar on tuber formation. 【Result】In the range of 4.0-9.0 min， the suitable sterilized time of 0.1% HgCl2 for terminal buds， the 2nd section axillary bud and the 3rd section buds were 5， 6 and 8 min， respectively. By adding 0-3.0 mg/L 6-BA in MS medium， the suitable primary medium for them were MS+1.0 mg/L 6-BA， MS+1.5 mg/L 6-BA and MS+2.0 mg/L 6-BA， respectively. By adding combination of 0-3.0 mg/L 6-BA and 0-0.4 mg/L PP333 in MS medium，MS+3.0 mg/L 6-BA+0.2 mg/L PP333 was beneficial for multiplication culture， and the highest multiplication coefficient was 7.0. In the range of 0-0.6 mg/L NAA， MS medium added with 0.4 mg/L NAA was more effective for rooting culture， and the highest rooting rate was 100.0%. In the range of 30.0-80.0 mg/L sugar， adding 60.0 g/L sugar in MS medium was appropriate for tuber formation， which resulted in 93.3% of tuberization rate.【Conclusion】The regeneration propagation system of purple-heart potato variety Black Beauty was established successfully to propagate virus-free plantlets and test-tubers. This tissue culture technology could be widely used in factory production of seed-tuber in Guangxi.