【Objective】The present study was conducted to analyze the genetic diversity of Diospyros germplasm resources in Guangxi by using SSR molecular marker in order to provide references for classification， identification， protection and utilization of Diospyros resource. 【Method】The 93 accessions of Diospyros germplasms collected from different eco-climatic regions of Guangxi were amplified by using 48 pairs of SSR primers， and then the cluster analysis and principal component analysis （PCA） were conducted to detect their genetic diversities and relationships. 【Result】Out of 48 pairs of primers， 15 pairs of primers could amplify 159 bands， and 154 of which were polymorphic loci with a polymorphism rate of 96.86%. The similarity coefficient between different germplasms varied from 0.478 to 0.925， with an average of 0.667. The cluster analysis conducted by unweighted pair-group method with arithmetic means （UPGMA） showed that， 93 accessions of Diospyros germplasms could be clusted into 4 groups at similarity coefficient of 0.640. The germplasms which belonged to Diospyros kaki Thunb， D. oleifera Cheng and D. lotus Linn were clustered into different groups. An overlapping phenomenon appeared in the genetic relationship between D. kaki var. silverstris Mak and Diospyros kaki Thunb. The results of PCA showed that， the contribution rate of the first three principal components were 14.18%， 5.25% and 4.11% in sequence， and the accumulated contribution rate was 23.54%. The PCA also could cluster 93 accessions of germplasms into 4 groups， which was similar to that of cluster analysis. 【Conclusion】The Diospyros germplasm resources in Guangxi present wide genetic background and abundant diversity， which can be used as important materials for utilization and breeding of Diospyros germplasm resources. SSR molecular marker can be used as a reliable tool for classification and identification of Diospyros germplasm resources.