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苦瓜种质资源苗期枯萎病抗性鉴定
作者: 陈振东1  2  3  黄如葵2  3  黎起秦1 *  文俊丽2  陈全艳1  梁家作2  
单位: (1 广西大学 农学院  南宁 530005;2 广西农业科学院 蔬菜研究所  南宁 530007;3 广西作物遗传改良生物技术  
关键词: 苦瓜  种质资源  枯萎病  抗性鉴定  
分类号:S436.429
出版年,卷(期):页码:2014 ,45 ( 10 ): 页码:1776-1780
摘要:

【目的】明确供试苦瓜种质资源对枯萎病尖孢镰刀菌苦瓜专化型(Fusarium oxysporum f. sp. Momordicae)的抗性,为抗病育种提供抗源。【方法】用苗期接种法对143份苦瓜高代自交系材料进行枯萎病抗性评价。【结果】在143份苦瓜种质资源中,具有高抗枯萎病特性的材料1份、抗病材料两份、中抗材料5份,分别占总数的0.70%、1.40%和3.50%;感病材料17份、高感材料118份,分别占总数的11.89%和82.52%;抗病材料主要来自中国大陆,果实性状为短棒形、皮色浅绿、瓜瘤为条瘤和条粒瘤的苦瓜资源。【结论】鉴定的苦瓜高代自交系材料普遍感枯萎病,加强苦瓜枯萎病抗性资源的收集引进、创新、鉴定和评价工作仍然十分必要。

【Objective】In order to provide anti-wilt source to the breeding program, bitter gourd germplasms were subjected to evaluation of the resistant level to the pathogen of Fusarium oxysporum f. sp. Momordicae. 【Method】143 inbred lines of bitter gourd were inoculated with the pathogen of Fusarium oxysporum f. sp. Momordicae, and the resistance to Fusarium wilt was identified according to the seedling-inoculation method. 【Result】The resistant levels to bitter gourd Fusarium wilt of all the germplasm materials were evaluated as 1 high resistance (HR), 2 resistance (R), 5 moderate resistance(MR), 17 susceptibility (S) and 118 high susceptibility (HS), accounting for 0.70%, 1.40%, 3.50%, 11.89% and 82.52%, respectively. The resistant germplasm mainly originated from mainland China, and their characteristics of fruit were short club in shape, light green in skin color, stripe or grain-stripe in fruit ribbing.【Conclusion】Most inbred lines of bitter gourd evaluated in this study were susceptible to Fusarium wilt. Therefore, more efforts need to be added to promote germplasm collection, identification and genetic improvement to excavate anti-wilt source to bitter gourd Fusarium wilt.

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