【Objective】The present experiment was conducted to study the influences of bamboo vinegar on seed germination and seedling growth of different flue-cured tobacco varieties in order to provide references for its application in tobacco production. 【Method】By using indoor culture dish method and greenhouse seedling-raising plate culture， four different bamboo vinegar dilutions （400， 200， 100， and 50 times） were designed and water was used as the control（CK）. The germination indices of Yunyan 97， Guiyan 2， Jiucaiping 2， Bina 1 and Honghuadajinyuan were determined after seed germinating for 14 days. The seedling growth indices of Yunyan 97 were investigated after 10 days of spaying bamboo vinegar on seedling leaves at the 3rd time. 【Result】Compared with the CK， different bamboo vinegar dilutions could inhibit the seed germination and seedling growth of all flue-cured tobacco varieties. The seed germination rate and index， whole length， root length， stem length and fresh weight of seedling for all varieties presented decreasing to varying degrees. The bamboo vinegar dilution of 50 times showed thorough inhibition to seed germination of all varieties. With the increase of bamboo vinegar concentration in culture dish， the seed germination rate and index， whole length， root length， stem length and fresh weight of seedling for all varieties showed decreasing trend. Excepting for variety Honghuadajinyuan， the sequence from low to high for seed vigor index of the other varieties was as follows: 100 times＜200 times＜400 times＜control， and different treatments showed significant difference. After spraying bamboo vinegar dilution at seedling stage， the stem girth of Yunyan 97 seedling in 200 times dilution treatment， the max leaf length in 50 times dilution treatment and the chlorophyll content in 100 times dilution treatment showed decreasing as compared to those of control. While the plant height， stem girth， the max leaf length and width， dry weight of seedling and root volume in different bamboo vinegar treatments were found to be increased to varying degrees. In all bamboo vinegar treatments， the plant height， max leaf length and root volume in 100 times dilution treatment were the highest with 3.36 and 10.14 cm， and 0.92 mL. And the stem girth， dry weight and max leaf width of seedling in 400 times dilution treatment also showed the highest with 1.82 cm， 0.262 g and 5.74 cm. 【Conclusion】Supplying 50-400 times bamboo vinegar solution in culture dish could inhibit the seed germination of flue-cured tobacco， while spraying 100-400 times bamboo vinegar solution to leaves could promote the seedling growth of Yunyan 97 to varying degrees.