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壳寡糖对干旱胁迫下甘蔗叶片生理指标的影响
作者: 刘晓霞1  邹成林2  3  李训碧1  周 桂1 *  
单位: (1 广西民族大学 海洋与生物技术学院/广西微生物与植物资源利用重点实验室  南宁 530006;2 广西作物遗传改良生物技术  
关键词: 壳寡糖  甘蔗  干旱胁迫  抗旱性  生理生化  
分类号:S566.101
出版年,卷(期):页码:2014 ,45 ( 10 ): 页码:1759-1763
摘要:

【目的】探讨干旱胁迫下壳寡糖对甘蔗生理指标的影响,明确壳寡糖的抗旱作用机制,为壳寡糖在作物抗旱中的应用提供参考依据。【方法】以台糖22号(ROC22)为材料,设正常供水(CK)、干旱处理、干旱胁迫条件下甘蔗叶片喷施100 mg/L壳寡糖溶液3个处理,在喷施壳寡糖后第2、4、6和8 d采集叶片样品,测定甘蔗叶片内肽酶、丙二醛(MDA)、可溶性糖、叶绿素荧光参数等。【结果】在正常供水条件下,甘蔗叶片内肽酶活性、MDA、可溶性糖含量、Fv / Fm和电子传递量子产量(ΦPSⅡ)、相对水分含量(RWC)均保持在一定水平,无明显变化。随着干旱胁迫时间的延长,干旱处理及喷施壳寡糖处理的甘蔗叶片内肽酶活性、MDA和可溶性糖含量呈上升的变化趋势,而干旱处理叶片Fv / Fm和电子传递量子产量(ΦPSⅡ)、RWC及壳寡糖处理的RWC呈不断下降的变化趋势。壳寡糖处理后第2~8 d的甘蔗叶片内肽酶活性不同程度高于干旱处理,而干旱处理第4~8 d的叶片MDA含量不同程度高于壳寡糖处理。经壳寡糖处理后第2~6 d叶片可溶性糖和RWC均稍低于干旱处理,但在第8 d显著高于干旱处理,分别为28.1 mg/g和66.7%。喷施壳寡糖处理后的叶片Fv / Fm和ΦPSⅡ呈不断增加的趋势,并在第8 d显著高于干旱胁迫处理,分别为干旱胁迫组的1.28和1.58倍。【结论】干旱胁迫下,外施壳寡糖可协调甘蔗叶片的生理代谢过程,改善甘蔗叶片对干旱胁迫的适应性,增强甘蔗的抗旱能力。

【Objective】The present study was carried out to explore the effects of oligochitosan treatment on sugarcane physiological indices and drought resistant mechanism under drought stress in order to provide  references for oligochitosan application in drought resistance of crops. 【Method】Sugarcane variety ROC22 was used as tested material. Three treatments viz., mormal watering (CK), keeping drought stress, and spraying 100 mg/L oligochitosan solution at the early elongation stage under drought stress were designed, and the +1 leaves of all treatments were taken on 2, 4, 6, 8 days after spraying to determine the endopeptidase, MDA, soluble sugar, Fv / Fm and ΦPS II. 【Result】Under normal watering, the endopeptidase activity, MDA content, soluble sugar content, Fv / Fm and ΦPS II, and RWC of sugarcane leaves kept at certain level without significant change. With the elongation of drought stress time, the endopeptidase activity, MDA and soluble sugar contents of sugarcane leaves in drought stress and oligochitosan treatments presented increasing trend. While the Fv / Fm and ΦPS II of drought stress treatment, and the relative water content(RWC) of drought stress and oligochitosan treatments showed decreasing trends. The endopeptidase activity of leaves after 2-8 days of oligochitosan treating was higher than that of drought stress treatment to varying degrees, while the MDA content of drought stress on 4-8 days was higher than that of oligochitosan treatment. The soluble sugar and RWC of leaves after 2-6 days of oligochitosan treating were lower than those of drought stress treatment, while they were significantly higher than the drought stress treatment on the 8th days, which were 28.1 mg/g and 66.7%. Meanwhile, the Fv / Fm and ΦPS II of oligochitosan treatment showed increasing trends and reached the highest on the 8th days, which were 1.28 and 1.58 times higher than drought stress treatment. 【Conclusion】It concluded that applying oligochitosan could regulate the process of physiological metabolism in sugarcane leaves under drought stress, improve adaptablity of sugarcane leaves to drought stress, and enhance drought resistance of sugarcane.

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