【Objective】The present experiment was conducted to investigate the effects of Cordycepsmilitaris Fjnu-01 fermentation liquor and mycelium powder on lifespan of Drosophila melanogaster, in order to provide scientific references for researching and developing new anti-aging products of Cordycepsmilitaris. 【Method】The different concentrations of Cordycepsmilitaris Fjnu-01 fermentation liquor and mycelium powder were adjusted to low-dose group（0.5%）, moderate-dose group（1.0%）, high-dose group（1.5%）, respectively. After Drosophila melanogaster fed with those different groups, the lifespan of Drosophila melanogaster were studied by determing maximum lifespan, average lifespan and life extending rates. 【Result】The results showed that Cordycepsmilitaris Fjnu-01 fermentation liquor and mycelium powder could significantly increased average and maximum lifespan of Drosophila melanogaster（P<0.01）. When adding fermentation liquor dosage of 1.5%, life extending rates of female and male were the highest, 78.78% and 74.86% respectively. When adding mycelium powder dosage of 1.5%, life extending rates of female and male were the highest, 71.18% and 68.57% respectively. 【Conclusion】Cordycepsmilitaris Fjnu-01 fermentation liquor and mycelium powder can significantly prolong lifespan of Drosophila melanogaster, and the effects of fermentation liquor is superior to that of mycelium powder. Meanwile, the effect on female is superior to effect on male.