【Objective】Two methods for observing early tobacco embryo cells were studied to evaluate their role in observing early tobacco embryo cells and provide references for research on tobacco embryo cells development. 【Method】Consistent observations on tobacco early embryo cells were made using whole transparency and enzymolysis isolation method. The merits and demerits of the two methods were analyzed. 【Result】Using the whole transparency method， two-celled proembryo， four-celled cell proembryo， and the undifferentiated globular embryo could be seen clearly， while the root suspensor of the early globular embryo was not clear and the embryo at the late stage was difficult to observe. Through the enzymolysis isolation method， the embryo at the whole stage could be observed （two-celled proembryo， the differentiated globular embryo， heart-shape embryo）. After twice enzymolysis separation， plasmolysis appeared from two-celled proembryo to the 16-celled early globular embryo， but it didn’t exist in late embryo stage. The results showed that the whole transparency method had many advantages such as easy to perform， less material required， in situ observation and so on. Using the enzymolysis isolation method， the process was shorter and embryonic cells could be formed excluding maternal tissues， but embryonic cells were lack of in situ. 【Conclusion】At tobacco embryonic development stage，suitable obserration method should be choosen according to the demands of different embryonic stage research，or two methods should be combined to observe early embryo for improving it’s continuous observation effect.