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薄壳山核桃MADS-box基因保守片段的克隆与序列分析
作者: 莫正海1  张计育1 *  翟 敏2  贾晓东1  宣继萍1  李永荣2  郭忠仁1 *  
单位: (1 中国科学院植物研究所  南京 210014;2 南京绿宙薄壳山核桃科技有限公司   南京 210072)  
关键词: 薄壳山核桃  MADS-box基因  RT-PCR  克隆  序列分析  
分类号:S664.1
出版年,卷(期):页码:2013 ,44 ( 5 ): 页码:730-734
摘要:

【Objective】The conservative fragment sequence of MADS-box genes from pecan was cloned and their phylogeny was assayed in order to provide references for cloning pecan new MADS-box family genes related to flower development and exploring molecular mechanism of pecan flower development. 【Method】The total RNA was extracted from male flower of pecan variety ‘Mahan’ and cDNA was synthesized using inverse transcription. The conservative fragment of MADS-box genes was cloned using RT-PCR, and the phylogenetic analysis was conducted using the deduced amino acid sequences with known Arabidopsis MADS-box gene subfamily. 【Result】Twenty-eight cDNA fragments of MADS-box gene were obtained by RT-PCR, and the length of these fragments was 137 bp including the initiation codon of genes. The homology of these nucleotide sequences was between 65.7% and 98.5%. The 11 deduced amino acid sequences were different. According to phylogenetic tree analysis, all these gene fragments involving different types of gene in ABCDE model were divided into the Arabidopsis MADS-box gene subfamily. 【Conclusion】There were many MADS-box genes in pecan. These gene fragments included all types of floral development genes in ABCDE model.

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