【Objective】The rapid propagation technique of Acacia auriculaeformis×Acacia mangium was studied to screen proper culture medium formulation for different stages and to achieve industrializing breeding. 【Method】Using hybrid Acacia stems as explants for tissue culture， effects of growth regulators including 6-BA， NAA and IBA of different concentrations on induced sprouting， subculture propagation and root induction were explored. 【Result】The results showed that in case of 0.2 mg/L NAA， the initial induction rate was 60.0% when no 6-BA was added， and the induction rate was 42.0% when there was0.2 mg/L 6-BA. At 0- 0.5 mg/L NAA， proliferation coefficient increased with the increase of 6-BA concentration. At 0.8 mg/L 6-BA， the propagation coefficient was 4.2. When 6-BA was above 1.0 mg/L， transgenerational seedling proliferation coefficient was 1.0. Using the improved MS as the basic medium， under the condition of 1.0 mg/L NAA， rooting induction rate was 17.5% and 24.0% for 0.5 and 1.0 mg/L IBA， respectively. Using the 1/2 modified MS as the basic medium， under the condition of 1.0 mg/L NAA， rooting rate was 91.7% and 50% for 0.5 mg/L IBA and 1.0 mg/L IBA， respectively. When rooting seedlings were transplanted in substrate with yellow subsoil and river sand or vermiculite mixture， transplanting survival rate was more than 85.0%. 【Conclusion】Improved MS +NAA 0.2 mg/L medium was more appropriate for the initial induction medium. Improved MS+6-BA 0.8 mg/L+NAA 0.5 mg/L was conducive to the growth of transgenerational seedlings. 1/2 modified MS+NAA 1.0 mg/L+IBA 0.5 mg/L was better for root induction.