【Objective】The present study was conducted to investigate effect of different dosages of fungal biofertilizer from Chaetomium globosum on growth，yield and quality of strawberry to provide references for utilizing the fungal biofertilizer in the field in future. 【Method】Zhangji strawberry and fungal biofertilizer from C. globosum were served as test materials. Percentage of colonization of endophytic fungus C. globosum ND35 was detected in strawberry. Effects of fungal biofertilizer with different dosages of C. globosum spores on biomassy，physiological quota of strawberry and production and quality of fruits were investigated and analyzed in the field and in laboratory. 【Result】Endophytic fungus C. globosum ND35 was able to colonize within plant of strawberry. Colonization rate was positively related to spores dosages of fungal biofertilizer. Different treatment of fungal biofertilizer from C. globosum significantly influenced growth，yield and quality of strawberry. Applying fungal biofertilizer at 0.5 g/plant and 1.0 g/plant was able to promote growth and development of strawberry. 【Conclusion】The 0.5－1.0 g/plant utilizing dosages of fungal biofertilizer from C. globosum was able to increase yield and improve quality of strawberry.