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茄子栽培种与水茄杂种无性系花粉发育及其对愈伤组织诱导的效应
作者: 王益奎1  2  3  方岩岩1  4  王 红1  3  4  李文嘉1 *  黎 炎1  
单位: (1 广西农业科学院 蔬菜研究所  南宁 530007;2 华南农业大学 园艺学院  广州 510642; 3 广西作物遗传改良生物技术  
关键词: 茄子  花药  愈伤组织  诱导率  
分类号:S641.1
出版年,卷(期):页码:2013 ,44 ( 12 ): 页码:1967-1971
摘要:

【Objective】The study explored the effects of different periods of pollen development and different explant pretreatment methods before innoculation to lay base for establishing the regeneration system of F1 clone of Solamum torvum (♂) × Solanum melongena(♀). 【Method】Using hybridal clone anther as explant, the relation between external form of flower bud and pollen development period was studied. The flower buds were disinfected at different concentrations of NaClO and HgCl2. The explants were placed at 4 ℃ and 36 ℃ for 2, 4, 6 and 8 d. The optimal pollen development period, disinfection and pretreatment methods were screened out. 【Result】The callus induction rate was the highest of 32.5% when the anther length was 5.6-7.3 mm and the microspore was at the middle and late uninucleate stage. The best disinfection treatment for F1 generation bud was firstly using 70% alcohol for 30s, then using 5% NaClO and 2 drops of Tween-20 for 15min, with the contamination rate of 4% and the browning rate of 4%. High-temperature pretreatment for 6d could markedly improve pollen callus induction rate. 【Conclusion】The best medium for initiation was anther length of 5.6-7.3 mm, flower bud length of 8.3-10.5 mm and using 70% alcohol for 30s, then using 5%NaClO and 2 drops of Tween-20 for 15 min and high-temperature pretreatment at 36 ℃ for 6d.

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