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甘蔗乙烯受体Sc-ERS启动子的克隆与序列分析
作者: 黄静丽1  牛俊奇1  朱 惠1  杨丽涛1  2  李杨瑞1  2  王爱勤1 *  
单位: (1 广西大学农学院  亚热带农业生物资源保护与利用国家重点实验室  南宁 530005;2中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所  农业部广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室  南宁 530007)  
关键词: 甘蔗  乙烯受体基因  启动子序列  克隆  
分类号:S566.1
出版年,卷(期):页码:2013 ,44 ( 5 ): 页码:722-729
摘要:

【Objective】The present study was conducted to clone the promoter sequence of sugarcane ethylene receptor (Sc-ERS) and analyze its acting elements. 【Method】Sc-ERS promoter sequence was cloned from the genomic DNA of sugarcane cultivar ROC22 with chromosome walking technology based on thermal asymmetric interlaced PCR principle, and the acting elements of Sc-ERS were analyzed with PlantCARE and PLACE online tools. 【Result】The gene promoter sequence of Sc-ERS was cloned, and it was 1410 bp in size sharing 82% and 80% homology with the sequences of ERS25 and ERS14 of corn, respectively. PlantCARE online analysis results showed that the gene promoter sequence of Sc-ERS had promoter acting elements TATA-BOX and CAAT-BOX, light-responsive elements, drought-induced MYB binding sites, methyl jasmonate-responsive element, salicylic acid response element, endosperm expression cis-regulatory elements, heat stress responsive elements and so on. 【Conclusion】A 1410 bp promoter sequence of Sc-ERS was cloned from sugarcane genomic DNA, which contained several specific acting elements, indicating that its expression could be regulated by circadian rhythm, hormone, drought, light and other factors.

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