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采用CFDA荧光染色技术监测木薯渣堆肥中的细菌动态
作者: 吴圣进1  2  韦仕岩1  王灿琴1  汪 茜1  覃晓娟1  陈廷速1  
单位: (1 广西农业科学院微生物研究所;2 广西作物遗传改良生物技术重点实验室:南宁 530007)  
关键词: 木薯渣  堆肥  CFDA技术  细菌  
分类号:S141.4
出版年,卷(期):页码:2012 ,43 ( 12 ): 页码:1976-1980
摘要:

【Objective】The present study aimed to determine the efficiency of CFDA fluorescent staining technique on detecting thermophilic bacteria in order to clarify the bacteria quantity changes during cassava residue compost and to provide a basis for regulating and evaluating the composing process. 【Method】Both thermophilic and mesophilic bacteria were detected by CFDA fluorescent staining under 30℃ and 60℃, and their staining effects were observed. Both the CFDA technique and plate count method were adopted simultaneously to monitor the dynamic changes in the quantity and vertical distribution of bacteria in cassava residue compost. 【Result】The CFDA technique could identify thermophilic bacteria effectively under 60℃. Data from CFDA technique showed that, during the cassava residue compost detection, mesophilic bacteria were quantitatively predominant at early stage, but decreased as the time of detection increased; on the other hand, the amount of thermophilic bacteria increased rapidly as time increased and became the predominant bacteria variety at the late fermentation stage. The highest temperature was detected when the compost pile measured at the depth of 10-40 cm, in which the thermophilic bacteria were predominant; in the bottom half of the compost pile, the temperature decreased, pH level turned acidic, and mesophilic bacteria became the predominant bacteria variety. 【Conclusion】Thermophilic bacteria were the predominant microbial group in the high temperature compost of cassava residue, and the CFDA technique could be used for the accurate evaluation of the bacteria group.

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