卢双楠1 2 李 粲3 滕 峥3 刘开雨3 刘 芳3 邱永福3 梁朝旭4 方位宽4
何姗珊4 刘晓静4 李 鸣2 4 * 梁 俊4 李容柏5 *
|单位： （1广西大学生命科学与技术学院 南宁 530005；2 广西作物遗传改良重点开放实验室 南宁 530007；|
|关键词： Cbcor15a 甘蔗 转基因 植物表达载体构建 瞬时表达|
|出版年,卷(期):页码：2012 ,43 ( 9 )： 页码：1262-1268|
【Objective】A new bivalent plant expression vector， named pCambia1300-cbcor15a-bar， was recombined by inserting two genes， i.e. Cbcor15a and eGFP， into the vector pCambia1300-bar and replacing the promoter CaMV 35s with Ubi-1. 【Method】Based on the multiple cloning sites of the expression vector pCambia1300-bar， the primers were designed according to the nucleotide sequence of gene Cbcor15a and eGFP and the promoter Ubi-1， and then the fragments of the genes and promoter were inserted into the vector pCambia1300-bar. The plasmids of the vector pCambia1300-cbcor15a-bar were transduced into onion epidermal cells via particle bombardment. The results were observed through fluorescence microscopy and confocal laser scanning microscope， respectively. 【Result】Compared to onion epidermal cell with pCambia1300-bar， onion epidermal cell transduced with the recombinant vector plasmid had a very bright green florescence. 【Conclusion】Downstream cold-induced gene Cbcor15a and reporter gene eGFP regulated by up-stream promoter Ubi-1 were expressed efficiently， which could ensure the construction of Cbcor15a.