【Objective】Ideal pineapple hybrid seeds germination medium and seedling production technology were explored for large-scale pineapple hybrid seed germination and seedling production. 【Method】Pineapple hybrid seeds， harvested from the cross Philippines variety （female parent） × Australian Smooth Cayenne （male parent） in July 2010， were cultured in various media including MS basic culture medium， filter paper and nutritious soil mixture to compare germination and growth of seedling. 【Result】Seedling emergence rate in MS medium treatment was recorded as the highest （30.9%） with vigorous seedlings. And transplant survival rate of these seedlings was 91.4%. Seedling emergence rate in filter paper treatment was only 19.1% with weak seedlings， and transplant survival rate was just 37.2%. Lastly， seedling rate of nutritious soil mixture treatment was 6.9% and transplant survival rate of seedling was 93.5%. 【Conclusion】It was concluded that MS culture medium with high germination rate， seedling emergence rate and transplant survival rate was optimal for pineapple hybrid seeds germination and seedling production.