【Objective】The present experiment was conducted to select Saccharum spontaneum L. clones with high combining ability and heritability and expand the sugarcane genetic diversity in breeding. 【Method】Seven Saccharum spontaneum L. clones， collected from different altitudes of Yunnan Province were selected by using SSR molecular marker and backcrossed with sugarcane variety Dezhe 93-94. The broad-sense heritability and combining ability of six main economic traits， including plant height，stalk diameter， brix， millable stalk number， sugar yield and cane yield were calculated by R software. 【Result】The results of variance showed that stalk diameter， sugar yield， cane yield， millable stalk number and plant height were highly significant， while the brix was significant. The broad-sense heritability of all combinations was excellent and found over 67.0%. The special combining ability of two combinations viz.， Dezhe 93-94×Yunge 82-114 and Dezhe 93-94×Yunnan 1 showed positive effects on plant height， millable stalk number， sugar yield and cane yield. The special combining ability of Dezhe 93-94×Ruige 07-3，Dezhe 93-94×Ruige 06-7-3 showed positive effects on stalk diameter， sugar yield and cane yield. The special combining ability of Dezhe 93-94×Yunge 83-184 and Dezhe 93-94×Yunge 83-174 showed positive effects on millable stalk number. 【Conclusion】The Saccharum spontaneum L. clones such as Yunge 82-114， Ruige 07-30， Ruige 06-7-3 and Yunnan 1， which were selected from low and middle altitude areas of Yunnan showed tremendous heritability and combining ability of cane and sugar yield， while Yunnan 1， Yunge 83-174 and Yunge 83-184 showed great heritability and combining ability of millable stalk number， therefore， may be used for developing innovative parents as required in breeding.