【Objective】Agricultural Resources and Environmental Research Institute (Guangxi Academy of Agricultural Sciences) and Guangxi Tianliyuan Biotechnology company collaborated to produce 25% organic bio-organic compound-mixed fertilizer with low N and high K content, which was used in field comparison test to observe its effects on the quality and yield of banana. 【Method】The effects of bio-organic compound-mixed fertilizer, imported compound fertilizer and common compound fertilizer with equivalent amount of N, P and K on the fruit yield and quality were determined, and production and economic performance of banana were analyzed. 【Results】Compared to imported and common compound fertilizer, bio-organic compound-mixed fertilizer increased banana fruit yield by 8.60 and 13.41%, net income by 8416.00 and 10858.00 Yuan/ha, soluble sugar content by 0.5 and 0.6 % and vitamin C by 3.0 and 4.0 mg/kg, respectively. 【Conclusion】The application of bio-organic compound-mixed fertilizer significantly enhanced the yield and its economic benefit, and improved the fruit quality.