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基质辅助激光解吸电离飞行时间质谱检测蛋白质分子量的优化研究
作者: 胡 炜1  2  付 强1  2  周 洁1  2  朱平川1  2  蒙 冰1  2  张 明1  2 *  
单位: (1 广西大学  南宁 530005;2 亚热带农业生物资源保护与利用国家重点实验室   南宁 530005)  
关键词: 基质辅助激光解吸电离飞行时间质谱   蛋白质   分子量   优化  
分类号:Q51.03
出版年,卷(期):页码:2012 ,43 ( 1 ): 页码:14-17
摘要:

【Objective】The optimum conditions for measuring protein molecular weight were studied using matrix assis-
ted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). 【Method】Three different matrices, viz., sinapic acid (SA), α-cyano-4-hydroxycinnamic acid (CHCA) and 2, 5-dihydroxybenzoic acid (DHB) were tested for determination of molecular weight of bovine serum albumin (BSA) and the effects of different sample concentration were observed on quantification of protein samples. 【Result】 The results demonstrated that 1 μg protein samples and sinapic acid (SA) matrix dissolved in water solvent (containing 0.1% trifluoroacetic acid and 50% acetonitrile to generate the concentration of 10 mg/mL) produced the best quality of MALDI-TOF-MS spectra. The sample required to determine the exact quantity was optimized as 500 ng. 【Conclusion】This technique is convenient, rapid, sensitive and reliable for determining protein molecular weight. The optimal combination was determined to obtain optimum mass spectra, necessary for obtaining accurate molecular weight of highly complicated protein samples.

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