<<登录旧系统审稿   登录新系统审稿>>

建议您登录自己的邮箱点击审稿链接进入审稿系统 ×
首页 学报新闻 学报概况 编委会 专家库 学术影响 投稿指南 审核流程 标准规范 下载专区 投诉邮箱 联系我们 EngLish

正文 您当前的位置:首页》正文

高山蓍嫩茎无性系的建立
作者: 于金凤  赵丹阳  王 娟  邹翠霞  姜长阳*  
单位: (辽宁师范大学生命科学学院  辽宁 大连 116029)  
关键词: 高山蓍  组织培养  无性系  快速繁殖  
分类号:S567.2
出版年,卷(期):页码:2012 ,43 ( 1 ): 页码:11-13
摘要:

【Objective】In order to protect wild germplasm resources and fulfill seedling demands for artificial cultivation, the present experiment was conducted to develop a regeneration system for Atractylodes japnica using young stem. 【Method】The young stem of A. japnica was used as explants to investigate the effects of different culture media and hormone combinations on callus induction and differentiation, rooting of adventitious buds, multiplication of test-tube plantlets and transplantation. 【Result】Among different culture media supplemented with different hormone combinations, viz., MS, 1/2MS, 8114 and B5,1/2MS+6-BA 0.8 mg/L mg/L+NAA 0.5 mg/L+2,4-D 1.5 mg/L and 1/2MS+6-BA 0.8 mg/L+NAA 0.5 mg/L+2,4-D 2.0 mg/L were optimum for callus induction, and 1/3MS and 1/3MS+ IAA 0.2 mg/L were ideal for callus differentiation. Of the 1/2MS, 1/4MS, N6 and B5, LS media supplemented with NAA (0.6 mg/L) and IAA (0.6 mg/L) and 1/4MS with IAA (0.3 mg/L) was optimum for rooting of adventitious buds and subculture multiplication of test-tube plantlets. The rooting rate was recorded as 92% and the average multiplication coefficient was 2.7. The test-tube plantlets were easy to be transplanted and survive, and showed 98.6% survival rate. 【Conclusion】The regeneration system for Atractylodes japnica was established and the transplanted plantlets grew vigorously, and flowered and fruited next year with all characteristics of wild plant.

基金项目:
作者简介:
参考文献:
服务与反馈:
文章下载】【加入收藏
总浏览数: 8 2 9 2 8 4 3  今日总人数: 7 7 1 3
主办:广西农业科学院    地址:广西南宁市大学东路174号   邮政编码:530007
电话:(+86)-771-3243905; (+86)-771-3244920   电子邮箱:nfnyxb@163.com
Copyright © 2011 Nfnyxb.com, All Rights Reserved  版权所有©2011《南方农业学报》编辑部   
 本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:13520358590

;