【Objective】The present experiment was conducted to improve the prevention ability of fowl adenovirus group-I vaccine in order to prevent the spread of fowl adenovirus group-I. 【Method】The virus with the titer of 106.3 TCID50/mL （107.58 copies/mL concentration of FAV-4） and titer of 106.9 TCID50/mL （108.82 copies/mL concentration of FAV-8） were used to prepare the vaccines. The inactivated oil-emulsion vaccines， viz.， univalent and bivalent were developed from fowl adenovirus group I serum type 4 and type 8 （FAV-4 and FAV-8） after optimization of virus replication conditions， and compared for inactivated effect of formaldehyde and β-propiolactone （BPL）. The vaccine characters were investigated， immune antibody level was monitored and the injecting virus test and culture test were carried out. 【Result】The lasting concentration of 1.00 mL/L formaldehyde and 0.500 mL/L β-propiolactone were found suitable for vaccine inactivation. Three vaccines showed white emulsion and could be preserved for 12 months at 4℃. The minimal dose of univalent and bivalent vaccines was 100 and 250 μL per chicken， respectively. The antibody level reached the OD450 value peak after 4 weeks of immunization and remained higher even after 8 weeks. Three vaccines showed 100% protection rate by protecting fowls from the attack of FAV completely after injecting virus. Under field conditions， antibody levels of three FAV oil-emulsion vaccines showed expected immune effects during initial immune stage and the immunity duration was over 6 months， the bivalent vaccine could reduce the injection time in production. 【Conclusion】It is concluded that the FAV-4 and FAV-8 bivalent oil-emulsion inactivated vaccines have good immune effects， long protection duration and good safety， and are very effective in reducing infection of fowl adenovirus group-I.