【Objective】The present experiment was conducted to investigate the effects of different concentration of gibberellic acid and ethephon on germination rate of mulberry seeds. 【Method】The seeds of three mulberry diploid hybrid combinations， viz.， Guisangyou 12， Guisangyou 62， Sha 2×Lun 109 and one triploid hybrid combination Sangteyou 1 were treated with different concentrations of gibberellic acid and ethephon （50， 100， 200 and 500 mg/kg） for 12 and 24 h to determine the germination rate. 【Result】 With the increase of gibberellic acid and ethephon concentration， the germination rate of seeds from four mulberry hybrid combinations increased initially， followed by a decrease. Three concentrations of gibberellic acid and ethephon （50， 100 and 200 mg/kg） increased the germination rate of three diploid mulberry varieties， while their 500 mg/kg concentration inhibited the germination of seeds. On comparing the duration of treatment， triploid hybrid seeds treated for 24 h with 50， 100， 200 and 500 mg/kg showed better germination compared to that of 12 h. 【Conclusion】In conclusion， the mulberry seeds soaked in 200 mg/kg gibberellic acid or ethephon for 24 h resulted in best germination rates of hybrid mulberry seeds.