【Objective】The present study was aimed to provide theoretical basis for establishing production technology system for kenaf male sterile line， maintainer line and hybrid seed production. 【Method】In this experiment， one-bud cut-grafting method was used. Buds obtained from overwintering branches of kenaf male sterile line 763A and maintainer line 763B were grafted on new overwintering branches of cotton rose （Hibiscus mutabilis L.） and kenaf wild variety H040， and then cutting in bed. The grafted cutting branches were transplanted in fields and their performance was compared with stock cuttings and scion seedlings. 【Result】The results are as follows：（1） The survival rate of grafting with male sterile line was significantly higher than that with maintainer line. When H040 was used as rootstock， the survival rate was recorded higher than others. When the cottonrose was used as rootstock， the survival rate was lower； （2）The grafted plants were dwarf， especially when the cottonrose was used as rootstock； （3） Grafting had little impact on the budding and flowering； （4） The withered top length of the plants was smaller than that of their stock plants when cottonrose and H040 were used as rootstock. The grafted plants showed strong cold resistance； （5） The number of fruits per plant was significantly and negatively correlated with the withered top length of the plants. The higher kenaf fruits were related with worse cold resistance. 【Conclusion】The effects of grafting kenaf with overwintering branches on growth and cold resistance of kenaf male sterile lines and maintainer lines were different. The grafted plants were dwarf. Appropriate rootstock could improve the cold resistance of grafted plants.