【Objective】The present experiment was conducted to induce corm in test tube plantlet derived from Zantedeschia hybrida to enhance propagation efficiency and transplant survival rate， shorten seedling raising time， reduce occurrence of serious disease and decrease high production cost. 【Method】The test tube plantlets with 3-7 cm height and 0.3 cm diameter were used as explants and inoculated to culture medium containing different sugar （2-8%）， paclobutrazol （1-8 mg/L） and chlorcholine chloride （2-16 mg/L）， the formation rate of corm was calculated after culturing for 90 days. 【Result】The formation rate of corm in Zantedeschia hybrida showed an increasing trend with increase in sugar in improved MS medium adding same paclobutrazol and chlorcholine chloride. With the increase of paclobutrazol and chlorcholine chloride in medium， the formation rate of corm increased initially， followed by a decrease. The induction effects of paclobutrazol （4 mg/L） and chlorcholine chloride （8 mg/L） was found the best. The highest corm formation rate was achieved in medium containing 4 mg/L paclobutrazol and 6% sugar. This combination also resulted in best average diameter and fresh weight of corm. The paclobutrazol and chlorcholine chloride had inhibitory effect on the growth of leaf， and differentiation and elongation of root. 【Conclusion】Adding suitable rate of sugar and paclobutrazol or chlorcholine chloride in improved MS+NAA （0.1 mg/L）+ agar （7 g/L） solid medium， can successfully induce the formation of corm in Zantedeschia hybrida， and the induction effect of paclobutrazol was better than that of chlorcholine chloride.