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广西45个主栽水稻品种抗性基因遗传多样性分析
作者: 张 晋1  2  罗志勇3  颜 群1  钟 波3  高利军1  2  潘筱凌3  赖海燕3  黄 鹂3  高汉亮1 *  郭小强3  
单位: (1广西农科院植物保护研究所  南宁 530007;2 广西作物遗传改良生物技术重点开放实验室  南宁 530007;  
关键词: 水稻  主栽品种  抗病基因同源序列  遗传背景  广西  
分类号:S511
出版年,卷(期):页码:2011 ,42 ( 10 ): 页码:1173-1176
摘要:

【Objective】The present experiment was conducted to assess the genetic diversity in relation to genetic variations in disease resistance amongst 45 main rice cultivars of Guangxi. 【Method】Five pairs of RGA primers were designed on the basis of already identified disease resistant genes in order to find out the presence or absence in 45 rice cultivars.【Result】Total 813 bands were amplified from 45 rice cultivars, of which 124 were polymorphic and the polymorphic rate was 76.07%. The cluster analysis result showed that the similarity coefficient of these cultivars ranged from 0.61 to 0.93 and these cultivars were divided into 5 classifications at 0.67. It also revealed the presence of five distinct indicating regionalism and distant genetic relationships amongst rice cultivar sources. 【Conclusion】Significant genetic variations have been observed in resistance to various diseases. Cultivar rotation and planting of mixed-cultivars with different genetic backgrounds have been suggested as an effective way for controlling rice blast disease in Guangxi.

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