【Objective】The studies were conducted to elucidate the causes of chlorosis in sugarcane in order to alleviate its effects and to develop some control measures. 【Method】Some soil physico-chemical characteristics such as pH and organic matter content and soil's nutrient status was analyzed during the early growth period of sugarcane planted in Nanning， Chongzuo and Laibin，Guangxi（China）. 【Result】The soils cultivated with sugarcane exhibiting chlorotic symptoms were found strongly acidic with higher levels of exchangeable Mn and available Al content， moderate levels of organic matter and total and available N， P and K content. Available Fe content of the soils was low， however，and the Fe content showed significant and positive correlation with the organic matters content of soils. 【Conclusion】The results of the present study indicated that lower pH and soil organic matter content and excessive available Al and exchangeable Mn may be associated with the chlorosis in sugarcane seedlings.