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壳寡糖诱导甘蔗叶多酚与防御酶活性的变化
作者: 周 桂1  靳晓芸1  邓光辉1  丘立杭2  3  邹成林1  李杨瑞2  3  4  5 *  
单位: (1 广西民族大学  南宁 530007;2 广西亚热带生物资源保护利用重点实验室;3广西大学农学院:南宁 530005;4 中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室;5广西作物遗传改良生物技术重点开放实验室:南宁 530007)  
关键词: 甘蔗  壳寡糖  多酚  苯丙氨酸解氨酶  多酚氧化酶  过氧化物酶  
分类号:S566.101
出版年,卷(期):页码:2011 ,42 ( 8 ): 页码:874-877
摘要:

【Objective】The present study was carried out to explore the effect of chitosan oligosaccharide on secondary metabolites and some defense enzyme activities related to stress resistance in sugarcane. 【Method】The leaves of sugarcane variety ROC22 were treated with 0 (CK), 25, 50 and 75 mg/L chitosan oligosaccharide solution at the early elongation stage. The polyphenol content and the activities of defense enzymes, viz., phenylalanine ammonialyase (PAL), polyphenol oxidase (PPO) and peroxidase (POD) were measured in +1 leaf of sugarcane at 1, 2, 3 and 4 d after spraying. 【Result】50-75 mg/L chitosan oligosaccharide treatment was conducive to the accumulation of polyphenol in sugarcane leaf. The PAL activity reached to its maximum and was 1.78 times higher than the control after 2 d of 50 mg/L chitosan oligosaccharide treatment. The PPO activity reached to its maximum and was 1.7 times higher than the control after 4 d of 50 mg/L chitosan oligosaccharide treatment. The 75 mg/L chitosan oligosaccharide treatment was conducive to improve POD activity of sugarcane leaf. 【Conclusion】It was found that 50-75 mg/L chitosan oligosaccharide treatment can improve the polyphenol accumulation and the activities of defense enzymes in sugarcane leaves, which is helpful for improving sugarcane resistance to various biotic and abiotic stresses.

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