【Objective】The germination and some chilling injury related with physio-biochemical indices of cassava were studied in order to forecast for application of control measures， and to evaluate the varieties tolerance to cold temperature. 【Method】Three cassava varieties， viz.， Guire 3， Huanan 124 and Huanan 205 were treated with different low temperatures （2， 5 and 8℃） for 0（CK），2， 4， 6， 8， 10， 12 and 14 d，respectively，in three replicates. The germination rate and some physio-biochemical indices such as relative conductivity and proline content， etc.， were determined. 【Result】Under the similar temperature conditions， the variety Huanan 124 showed maximum germination rate followed by the varieties Huanan 205  and Guire 3. However， the relative conductivity of Guire 3 increased sharply， compared to Huanan 124， which was comparatively slower at the early stage of the treatment. The relative conductivity of Hunan 205 treated for 6 d at 2℃ was over 6%. The proline content of Guire 3， Huanan 205 and Huanan 124 were found more than 80.0% in the treatments of 4， 6 and 12 d， respectively， at 2℃ temperature. 【Conclusion】The relative conductivity and proline content may be used as physio-biochemical markers for evaluating cassava varieties against low temperature.