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青岛大花脱毒种苗快繁技术研究
作者: 张梅秀  魏玉杰  臧广鹏  杨振华  于红霞*  
单位: (甘肃省农垦农业研究院  甘肃武威 733006)  
关键词: 啤酒花  青岛大花  脱毒苗  快繁  单芽茎段  
分类号:S571.9
出版年,卷(期):页码:2011 ,42 ( 2 ): 页码:128-130
摘要:

【Objective】The present experiment was conducted to study the regeneration technologies for virus-free plantlet of Humulus lupulus L. variety Qingdaodahua in order to develop a convenient method for its rapid propagation.【Method】The single budded stem of Humulus lupulus L. variety Qingdaodahua was used as explants to investigate the effects of culture medium containing water from different sources (tap water, cool boiling water, distilled water and local well water), residual root stubble and simple nutrient solution (root stubble with single bud, root stubble +simple solution as MS medium without hormone, new culture medium), and different hormone combinations (0.01-0.1 mg/L 6-BA and 0.02-0.2 mg/L IAA) on bud induction and shoot regeneration. 【Result】The results showed that the effects of local well water on explants culture was better than the other water types, the higher rooting stem number, rooting rate, average root length, shoot diameter and height were observed in the medium containing water from the local well, however, the induction and proliferation of buds and proliferation time were found to be lesser. This was followed by the medium containing cool boiling water. The rooting stem number, induction buds, proliferation buds and times, and shoot height of explants in tap water medium were lower than that of the control of distilled water. After culturing the explants for 20 days in residual root stubble medium+20 mL simple solution, the induction and proliferation of buds, proliferation time and shoot height were found higher than those in new culture medium, and the leaves appeared on the shoots were in deep green. With the increase of 6-BA and IAA concentration in medium, the induction of buds, rooting rate and time of single bud proliferation decreased initially, and then registered an incease, and these were highest in the medium containing combination 6-BA (0.1 mg/L) and IAA (0.2 mg/L). 【Conclution】For the induction of buds in explant and regeneration of shoot, local well water or cool boiling water may be used as a substitute of distilled water in culture medium. The subculture medium and residual stubble can be used continuously to culture explants by adding simple solution (MS without hormone). Lower concentrations of hormone combination 6-BA (0.01 mg/L)+IAA(0.02 mg/L) can be used to induce the buds and regeneration of shoot derived from virus-free plantlet of Humulus lupulus L. variety Qingdaodahua.

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