【Objective】The experiment was conducted in order to standardized the pollution-free cultivation techique for Dendrobium officinale Kimura et Migo and  the standards of product quality were analyzed. 【Method】The wild red skin soft foot （Dendrobium officinale Kimura et Migo） plants were tissue cultured to investigate the influence of different qualities （high and qualified） of seedling and different cultivation methods （bedstead planting， growth chamber planting） on its growth， growth vigor and the yield and grade of dendrobine and polysaccharide in fresh stem. 【Result】Different cultivation methods did not greatly affect the survival rate of Dendrobium officinale plantlets， however， the growth vigor， growth mass and stem yield of seedlings planted in bedstead method were superior to those planted in growth chambers. The survival rate， growth vigor and stem yield were found higher in plants raised from high-quality seedling. Organophosphorus pesticide residues were present in bedstead production， while it was absent in plants cultivated in growth chambers. The polysaccharide content in whaite shoot， green shoot and top grade trunk were 19.72，18.54 and 29.41%， respectively. Up to 2.5 years of growth stage， dendrobine content in plant were found to increase with increasing years of planting. 【Conclusion】The bedstead planting using fine quality seedling with eco-friendly control measures of insect pests was standardized to ensure the pollution-free Dendrobium officinal cultivation in Guangxi. The major identification index of Dendrobium officinale product quality was determined as follows: the polysaccharide contents in fresh stem≥18%， the polysaccharide contents in Dendrobium candicum≥25%， and heavy metal content and pesticide residues should not exceed to the specified index.